产地：ABC
货号：ABC111647
规格：100UL
备注：
| 产品名称 | 产品编号 | 规格 |
| Anti -Collagen III Rabbit pAb | ABC111647 | 100UL |
| Protein full name | Collagen alpha-1(III) chain |
| Synonyms | COL3A1, EDS4A, collagen type III alpha 1, collagen type III alpha 1 chain |
| Immunogen | Recombinant protein corresponding to Mouse Collagen III |
| Uniprot ID | P08121 |
| Isotype | IgG |
| Purity | Affinity purification |
| Subcellular location | Secreted |
| Predicted MW. / Observed MW. | 138 kDa / 140-220 kDa |
| WB | Mouse, Rat | 1: 500-1: 1000 | skin |
| IHC/IF | Human, Mouse, Rat | 1: 250-1: 1000 | kidney, liver, lung |
This gene encodes the pro-alpha1 chains of type III collagen, a fibrillar collagen that is found in extensible connective tissues such as skin, lung, uterus, intestine and the vascular system, frequently in association with type I collagen. Mutations in this gene are associated with Ehlers-Danlos syndrome types IV, and with aortic and arterial aneurysms. Two transcripts, resulting from the use of alternate polyadenylation signals, have been identified for this gene.
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WB analysis of collagen III (GB111629). Sample: Protein treated by RIPA Lysis Buffer (G2002). Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h. Primary antibody: 1: 1000, 4℃ overnight. Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 3000, RT, 1h. |
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IHC analysis of collagen III (GB111629). Sample: Human kidney (Paraffin), 4% PFA (G1101) 12-24h. Antigen retrieval: Pepsin Antigen Repair Solution,37℃,30min. Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min. Primary antibody: 1: 500, 4℃ overnight. Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 200 RT 1h. |
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IHC analysis of collagen III (GB111629). Sample: Rat kidney (Paraffin), 4% PFA (G1101) 12-24h. Antigen retrieval: Pepsin Antigen Repair Solution,37℃,30min. Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min. Primary antibody: 1: 1000, 4℃ overnight. Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 200 RT 1h. |
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IHC analysis of collagen III (GB111629). Sample: Rat lung (Paraffin), 4% PFA (G1101) 12-24h. Antigen retrieval: Pepsin Antigen Repair Solution,37℃,30min. Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min. Primary antibody: 1: 500, 4℃ overnight. Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 200 RT 1h. |
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IF analysis of collagen III (GB111629). Sample: Mouse kidney (Paraffin), 4% PFA (G1101) 12-24h. Antigen retrieval: Pepsin Antigen Repair Solution,37℃,30min. Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min. Primary antibody: 1: 1000, 4℃ overnight. Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300 RT, 1h. |
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IF analysis of collagen III (GB111629). Sample: Mouse lung (Paraffin), 4% PFA (G1101) 12-24h. Antigen retrieval: Pepsin Antigen Repair Solution,37℃,30min. Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min. Primary antibody: 1: 1000, 4℃ overnight. Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300 RT, 1h. |
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IF analysis of collagen III (GB111629). Sample: Rat liver (Paraffin), 4% PFA (G1101) 12-24h. Antigen retrieval: Pepsin Antigen Repair Solution,37℃,30min. Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min. Primary antibody: 1: 1000, 4℃ overnight. Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300 RT, 1h. |
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IF analysis of collagen III (GB111629). Sample: Rat lung (Paraffin), 4% PFA (G1101) 12-24h. Antigen retrieval: Pepsin Antigen Repair Solution,37℃,30min. Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min. Primary antibody: 1: 1000, 4℃ overnight. Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300 RT, 1h. |
| Storage | Store at -20 ℃ for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.
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