产地:ABC
货号:ABC11156
规格:100UL
备注:
产品名称 | 产品编号 | 规格 |
Anti -NeuN Rabbit pAb | ABC11156 | 100UL |
Protein full name | RNA binding protein fox-1 homolog 3 |
Synonyms | FOX3, HRNBP3, NeuN, RBFOX3 |
Immunogen | KLH conjugated Synthetic peptide corresponding to Mouse NeuN |
Uniprot ID | Q8BIF2 |
Isotype | IgG |
Purity | Affinity purification |
Subcellular location | Nucleus, Cytoplasm |
Predicted MW. / Observed MW. | 41 kDa / 48 kDa |
Applications | Species | Dilution | Positive Sample |
WB | Mouse, Rat | 1:500-1:1000 | brain, cortex, hippocampus |
IHC/IF | Human, Mouse, Rat | 1:500-1:1000 | brain, hippocampus |
NeuN, encoded by FOX3, is a neuron-specific nuclear protein. Anti-NeuN stains exclusively neuronal cells in the central and peripheral nervous systems, especially postmitotic and differentiating neurons, as well as terminally differentiated neurons. Anti-NeuN has been used widely as a reliable tool to detect most postmitotic neuronal cell types. The immunohistochemical staining is primarily localized in the nucleus of the neurons with lighter staining in the cytoplasm.
WB analysis of Neun (GB11138). Sample: Protein treated by RIPA Lysis Buffer (G2002). Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h. Primary antibody: 1: 1000, 4℃ overnight. Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 3000, RT, 1h. |
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IHC analysis of neun (GB11138). Sample: Rat brain (Paraffin), 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201),98℃,20 min. Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min. Primary antibody: 1: 1000, 4℃ overnight. Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 200 RT 1h. |
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IHC analysis of neun (GB11138). Sample: Mouse brain (Paraffin), 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201),98℃,20 min. Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min. Primary antibody: 1: 500, 4℃ overnight. Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 200 RT 1h. |
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IF analysis of neun (GB11138). Sample: Rat hippocampus (Paraffin), 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201),98℃,20 min. Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min. Primary antibody: 1: 500, 4℃ overnight. Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300 RT, 1h. |
Storage | Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles. |
Storage Buffer | PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol. |
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer (G2025).
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