产地：ABC
货号：ABC11156
规格：100UL
备注：
| 产品名称 | 产品编号 | 规格 |
| Anti -NeuN Rabbit pAb | ABC11156 | 100UL |
| Protein full name | RNA binding protein fox-1 homolog 3 |
| Synonyms | FOX3, HRNBP3, NeuN, RBFOX3 |
| Immunogen | KLH conjugated Synthetic peptide corresponding to Mouse NeuN |
| Uniprot ID | Q8BIF2 |
| Isotype | IgG |
| Purity | Affinity purification |
| Subcellular location | Nucleus, Cytoplasm |
| Predicted MW. / Observed MW. | 41 kDa / 48 kDa |
| Applications | Species | Dilution | Positive Sample |
| WB | Mouse, Rat | 1:500-1:1000 | brain, cortex, hippocampus |
| IHC/IF | Human, Mouse, Rat | 1:500-1:1000 | brain, hippocampus |
NeuN, encoded by FOX3, is a neuron-specific nuclear protein. Anti-NeuN stains exclusively neuronal cells in the central and peripheral nervous systems, especially postmitotic and differentiating neurons, as well as terminally differentiated neurons. Anti-NeuN has been used widely as a reliable tool to detect most postmitotic neuronal cell types. The immunohistochemical staining is primarily localized in the nucleus of the neurons with lighter staining in the cytoplasm.
|
WB analysis of Neun (GB11138). Sample: Protein treated by RIPA Lysis Buffer (G2002). Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h. Primary antibody: 1: 1000, 4℃ overnight. Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 3000, RT, 1h. |
|
IHC analysis of neun (GB11138). Sample: Rat brain (Paraffin), 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201),98℃,20 min. Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min. Primary antibody: 1: 1000, 4℃ overnight. Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 200 RT 1h. |
|
IHC analysis of neun (GB11138). Sample: Mouse brain (Paraffin), 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201),98℃,20 min. Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min. Primary antibody: 1: 500, 4℃ overnight. Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 200 RT 1h. |
|
IF analysis of neun (GB11138). Sample: Rat hippocampus (Paraffin), 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201),98℃,20 min. Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min. Primary antibody: 1: 500, 4℃ overnight. Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300 RT, 1h. |
| Storage | Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol. |
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.
爱必胜生物科技(东莞)有限公司 © 版权所有
技术支持：网一科技