产地：ABC
货号：ABC12353
规格：100UL
备注：
| 产品名称 | 产品编号 | 规格 |
| Anti -Insulin Mouse mAb | ABC12353 | 100UL |
| Protein full name | Insulin-1 |
| Synonyms | INS, IDDM, IDDM1, IDDM2, ILPR, IRDN, MODY10, insulin |
| Immunogen | Recombinant protein corresponding to Mouse Insulin |
| Uniprot ID | P01325 |
| Isotype | IgG2a,κ |
| Purity | Affinity purification |
| Subcellular location | Secreted |
| Predicted MW. / Observed MW. | 12 kDa / 12 kDa |
| WB | Mouse | 1: 1000-1: 2000 | pancreas |
| IHC/IF | Human, Mouse, Rat | 1: 1000-1: 2000 | pancreas |
Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver.
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WB analysis of insulin (GB12335). Sample: Protein treated by RIPA Lysis Buffer (G2002). Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h. Primary antibody: 1: 1000, 4℃ overnight. Secondary antibody: HRP Goat Anti-Mouse lgG (GB23301), 1: 3000, RT, 1h. |
|
IHC analysis of insulin (GB12335). Sample: Mouse pancreas (Paraffin), 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2min. Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min. Primary antibody: , 4℃ overnight. Secondary antibody: HRP Goat Anti-Mouse lgG (GB23301), 1: 200 RT 1h. |
|
IF analysis of insulin (GB12335). Sample: Human pancreas (Paraffin), 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2min. Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min. Primary antibody: 1: 500, 4℃ overnight. Secondary antibody: Cy3 conjugated Goat Anti-mouse IgG (H+L)(GB21301),1: 200 RT 1h. |
|
IF analysis of insulin (GB12335). Sample: Mouse pancreas (Paraffin), 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2min. Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min. Primary antibody: 1: 500, 4℃ overnight. Secondary antibody: Cy3 conjugated Goat Anti-mouse IgG (H+L)(GB21301),1: 200 RT 1h. |
|
IF analysis of insulin (GB12335). Sample: Rat pancreas (Paraffin), 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2min. Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min. Primary antibody: 1: 500, 4℃ overnight. Secondary antibody: Cy3 conjugated Goat Anti-mouse IgG (H+L)(GB21301),1: 200 RT 1h. |
| Storage | Store at -20 ℃ for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.
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