单克隆抗体_ABC12069-100UL Anti -BrdU Mouse mAb

Anti -BrdU Mouse mAb （ABC12069）

价格：1200.0

产地：ABC

货号：ABC12069

规格：100UL

备注：

产品信息

产品名称	产品编号	规格
Anti -BrdU Mouse mAb	ABC12069	100UL

Product Information

Protein full name	Bromodeoxyuridine
Synonyms	Bromodeoxyuridine, Bromodeoxyuridine,5-Bromo-2′-deoxyuridine; 5-BrdU, Proliferation Marker; 5-Bromo-2-deoxyUridine
Immunogen	KLH conjugated Synthetic peptide corresponding to Mouse BrdU
Isotype	IgG1,κ
Purity	Affinity purification
Subcellular location	Nucleus

Applications

IHC/IF

All species

1: 100-1: 500

jejunum, neonatal mouse's brain

Background

Bromodeoxyuridine (5-bromo-2'-deoxyuridine, BrdU, BUdR, BrdUrd) is a synthetic nucleoside that is an analog of thymidine. It can be incorporated into the newly synthesized DNA of replicating cells (during the S phase of the cell cycle), substituting for thymidine during DNA replication. As such, BrdU is used for birth dating and monitoring cell proliferation. BrdU is a toxic and mutagenic substance. It triggers cell death, the formation of teratomas, alters DNA stability, lengthens the cell cycle, and has mitogenic, transcriptional and translational effects on cells that incorporate it.

Images

	IHC analysis of Brdu (GB12051). Sample: Mouse jejunum (Paraffin), 4% PFA (G1101) 12-24h. Antigen retrieval: 2M HCL in pure water，37℃, 30minBlocking buffer: 3% BSA in PBS (GC305010), RT, 30min. Primary antibody: 1: 500, 4℃ overnight. Secondary antibody: HRP Goat Anti-Mouse lgG (GB23301), 1: 200 RT 1h.
	IHC analysis of Brdu (GB12051). Sample: Mouse jejunum (Paraffin), 4% PFA (G1101) 12-24h. Antigen retrieval: 2M HCL in pure water，37℃, 30minBlocking buffer: 3% BSA in PBS (GC305010), RT, 30min. Primary antibody: 1: 500, 4℃ overnight. Secondary antibody: HRP Goat Anti-Mouse lgG (GB23301), 1: 200 RT 1h.
	IF analysis of Brdu (GB12051). Sample: Mouse neonatal mouse's brain (Paraffin), 4% PFA (G1101) 12-24h. Antigen retrieval: TE buffer (pH 9.0) (G1203),98℃,20min. Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min. Primary antibody: 1: 500, 4℃ overnight. Secondary antibody: Cy3 conjugated Goat Anti-mouse IgG (H+L)(GB21301),1: 200 RT 1h.

Storage

Storage	Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles.
Storage Buffer	PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol.

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.

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